application of polymerase chain reaction for identification of dermatophytes

. This study was carried out on a total of 35 isolates related to 6 species of dermatophytes which were isolated from animal and human on SDA and DTM.The source for human isolates was Misr University for Science and Technology, 6 October Governorate ,and for animals isolates military and privte farms in Belbis and Salhia in Sharkia Gavernorate .The main objective of the current research was done to study the genotypic method for identification of isolated dermatophytes comparing it with the phenotypic method .Phenotypic identification carried out by macroscopy and microscopic examination of isolates,as well as by phenotypical characters on BCP-MSGA,LRA and PDA .Macroscopic and microscopic examination of the cultures were done to observe the surface and reverse colour, consistency, texture, presence of pigments.Microscopic characters were examined after using LPCB to demonstrate the presence of hyphae and it’s modification, macroconidia, microconidia, chlamdospores and other fungal structure. Phenotypical characters were examined by change on color and hydrolysiGentotypic identification applied on 35 isolates by extraction of DNA from 10 strains of Microsporum canis by using phenol-chloroform isoamylalcohol (CTAB) and for the other 25 strains using Nexttec kit clean columns of yeast.PCR-ITS were done for 35 isolates by using V9D and mass 266 universal primers, samples were run on 1.4% agarose gel at 140 volt for one hour. The expected band was at 1031 bp without any contamination.For M. .