The hepatotoxic effects of lambada cyhalothrin insecticide in vivo and in vitro using isolated rat hepatocytes

Pesticides are occasionally used in large amounts causing environmental pollution and therefore, are a cause of concern. Synthetic pyrethroids are broad spectrum insecticides active tovarying degree on an extensive range of arthropod species and used widely against insect pests in agriculture, veterinary and domestic outlets. Lambda-cyhalothrin (LCT) is one of the type 11 newer synthetic pyrethroid insecticides with an effective, immedpersistent activity against a large variety of arthropods harmful bothto human and animal health and to vegetal production. from the previous studies there is no enough data on thehepatotoxicity of LCT. Therefore, the present study aimed to investigate the hepatotoxicity of LcT either in vitro (for technical grade) or in vivo (for both technical grade and commercial 2.5%) beside an in vitro mechanistic study to elucidate the role omicrosomal enzymes in the LCT hepatotoxicity using inducers andinhibitors models in freshly isolated male rat hepatocytes. I. In vitro hepatotoxicity study involves two experiments as following: The first experiment involved determination of cytotoxicity and oxidative stress induced by LCT in isolated rat hepatocytesincubation medium) and time points of incubation (0, 30, 60 and 120 minutes). Cytotoxicity was determined by assessing of cell viability and hepatic enzymes (LDH, ALT ”and AST) leakagespercentage. Oxidative stress induced by LCT was measured by GSH content and TBARS accumulation. The results of thisexperiment revealed the LCT exhibited concentration and time-