The Effect of adipose derived stem cells on bone healing in experimental animals

Adipose derived mesenchymal stem cells (ADMSCS) have been continuously comeinto focus of interest as an easyaccessible source for pluripotent cell. To date theuse of modified well defined canine ADMSCs (cADM5Cs) in regenerative veterinary medicine has been limited. This is attributed to a missing defined characterization concept of pluripotent marker gene expression. A total of 22 subcutaneous fat samples (apprunderwent different surgeries without any other evident disease. Herein, we present isolation and characterization of un-diferentiated cADMSCs by a marker set for gene and protein expression confirming the pluripotentcharacteristics of the cells by quanticADMSCs showed spindle fibroblst-like morphology. Positive gene expression ofOCT4, SOX2, KLF4, cMYC and NANOG was confirmed by qPCR. Additionally,cADMSCs showed positive expression of MSC - specific markers C090 and COlDS, as well as embryonic stem cell -specific surface markers (stage-specific embryonic antigens SSEA-3 and SSEA-4), while the expression of the hematopoietic marker eD4S was lacking medium. The demonstrated results allow for future investigations of marker gene and protein characteristics of cADMSCs e.g. in context with gene therapeutic modifications or external protein application of the cells.