Pathological and Immunohistochemical Studies on The Effect of Synthetic Glucocorticoids Compounds on Rabbits

The present work is performed to study the pathological andimmunohistochemical alterations after administration of(prednisolone) for rabbits.Twenty seven, New Zealand, male white, rabbits of four monthsold and 1500 gm B.wt. were used in this work. They were dividedinto 2 groups. Group I, it contains 18 and each rabbit was given orallyby stomach tube 4.2 mg prednisolone as (initial dose) daily for 45days. Six rabbits were sacrificed after 45 days post treatment(subgroup A). The remaining 12 rabbits were given 2.8 mgprednisolone as (maintenance dose) daily for extra 30 days. Sixrabbits were sacrificed after 75 days post treatment (subgroup B).Later on, the remaining 6 rabbits were also received 0.7 mgprednisolone as (withdrawal dose) daily for extra 30 days. Theremaining 6 rabbits from group I were sacrificed 105 days posttreatmentIsubgroup C).Group II (9 rabbits) were kept as a control group and 3 rabbitswere sacrificed on 45, 75 and 105 days.The main clinical signs among rabbits of (subgroup A) werepolydipsia and polyuria. Liver revealed congestion, hemorrhage,programmed and accidental cells death (coagulative necrosis) .Kidneys showed contracted glomerular tufts, hyDROPic degenerations,perivascular edema and extravasated erythrocytes. Spleen exhibitedlymphoid depletion in some white pulps with extensivehemosiderosis. Heart revealed congestion, cloudy swelling and