| Abstract: |
Summary :-A total of 60 samples were taken from the outer surfaces of shoulder and thigh of cattle carcasses, slaughtered, skinned, eviscerated, prepared and post-mortem examined at Zagazig traditional abattoir just before stamping and passed as fit for h* The samples were divided to two categories:Thirty samples were taken from the outer surfaces of shoulder region of cattle carcasses. Samples were collected using both of swab and surface tissue excision techniques, 15 samples with each just before stamping and without any treatment, the same techn* The results revealed that:-a) The mean value of Enterobacteriaceae count/ cm2 and most probable number of Coliforms (MPN) of shoulder outer surface by swab method were 5.7x103 and 4.8x102 organisms/cm2, respectively; such values by tissue surface excision were 3.3x105 and 1.2x103 ob) The mean value of Enterobacteriaceae count/ cm2 and most probable number of Coliforms (MPN) of shoulder outer surface by swab method were 1.6x103 and 3.3x102 organisms/ cm2, respectively; such values by tissue surface excision were 6.3x104 and 1.4x103 The achieved results proved that tissue surface excision gave higher microbial counts than that of swab method.Furthermore, the members of Enterobacteriaceae recovered from the examined samples were E. coli, Enterobacter agglomerans, Citrobacter diversus, Citrobacter amalonaticus, Serratia fonticola, Kluyvera ascorbata, Kluyvera cryocrescens, and Klebsiella pneumoThe total Enterotoxigenic E.coli and Enteropathogenic E.coli were 10 strains. ETEC (O78) constituted 70%, while EPEC (O86) constituted 30%. All ETEC were isolated from shoulder by excision method, while EPEC were 20% from shoulder and 10% from thigh by thThe public health importance of existed microorganisms as well as the recommended methods to improve the sanitary status of carcasses and to prevent contamination was discussed.
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