Evaluation of Some Molecular and Immunological Techniques Used for Detection of virulence Genes of Bacteria Causing Mastitis In Cattle

Summary:-Mastitis is the inflammatory response of the mammary gland to noxious agents that can be either infectious or non infectious. Most frequently the aetiology is infectious organisms such as S.aureus and E.coli which have been implicated as causes oThe innate udder defense plays a role to protect the healthy gland in a normal non-infected and noninflamed quarter. The somatic cell population consists of polymorphonuclear cells, macrophages and lymphocytes, while the mammary gland contains nonspecificThis study was planned as an attempt to throw spot lights upon bovine bacterial mastitis with special reference to that caused by S.aureus and E.coli. For attaining this purpose the following points were undertaken:1- A number of 106 milk samples were collected from mastitic cows.2- Culture and biochemical identification were done for the isolatedS.aureus and E.coli strains.3- API20 E Kits was used for biochemical identification of isolated E.colifrom mastitic milk samples.4- Detection of virulence factors of isolated E.coli by .