Studies on Malassezia Species Isolated from Human and Animal With Special Reference to the New Methods of Identification

Summary:-Yeast of genus Malassezia is a part of the human skin and worm blooded animal’s flora. Malassezia species lipophilic unipolar yeast recognized as commensal of the skin that may be pathogenic under certain conditions. This lipophilic yeast associaA total of (90) samples have been collected from both diseased human, diseased and apparently healthy animals. Human samples were collected from cases of tinea versicolor and seborrheic dermatitis as skin scrapings while animal samples were collected fromAs regard to human samples, (14) Malassezia isolates were obtained from (20) samples which identified into (3) Malassezia species namely M. furfur 9 (64.28%), M. sympodialis 4 (28.57%) and M. globosa 1(7.1%).Concerning to 70 samples collected from apparently healthy and diseased animals, 15 Malassezia isolates were obtained from them 13 M. pachydermatis with frequency of (86.67%) and 2 M. furfur with (13.3%).The isolates were identified into Malassezia species by using the phenotypic method which including morphological characteristics (macroscopicmorphology and microscopicmorphology) and physiological characteristics of the isolated Malassezia species. MacroMalassezia isolates were subjected for antifungal sensitivity test using Itraconazole, Ketoconazole, Fluconazole, Clotrimazole, Nystatin and Amphotericin. Antifungal sensitivity test for M. furfur, M. sympodialis, M. globosa and M. pachydermatis, which ob