This study comprised the examination of seven hundred andeighteen samples that were collected from farms and backyard of ducksin Dakahlia and Sharkia Govemorates to detect the incidence of naturalinfection of ducks with Mycoplasma. The isolates were identified byphenotypic and genotypic. Mycoplasma gallisepticum (MG) wasisolated from (76.92%) of examined lungs, (33.33%) of egg yolk and(25%) of trachea. Also M. gallinarum was isolated from (75%) oftrachea, (36.36%) of tracheal swabs. While, M. anatis was isolated from(66.67% and 23.08%) of egg yolk and lungs, respectively. Acholeplasmaspecies could be isolated only from (63.64%) of tracheal swabs.