| Abstract: |
Fifty freshly dead and /or diseafrom different localities at Sharkia Governsed young rabbits were collected orate. The collected rabbits were subjected to clinical and/or pathological examination at the Departement of Avian and Rabbit Medicine, Faculty ofTrials for bacterial isolation and identification were carried out on the examined rabbits. Clostridium perfringens, E-coli, Klebsiella and Citrobacter could be isolated with a percentage 62%, 32%, 12% and 8% respectively.The seasonal variation among the examined rabbits were recorded, C. perfringens and Citrobacter in winter season were 80% and 20%, respectively. E-coli in spring and winter seasons was 40% in both seasons, while klebsiella in autumn season was 17.64%.Age variation among the examined rabbits were reported, C. perfringens, Klebsiella and Citrobacter at 2-3 months old rabbits were 70.58%, 17.64% and 11.76%, respectively, E-coli at 1-2 months old rabbits was 61.1%.The bacterial isolates were biotyped as 31 C. Perfringens, 16 E-coli, 6 Klebsiella and 4 Citrobacter species isolates.Serotyping of nine E-coli isolates revealed six O E-coli serogroups (O26, O114, O44, O128, O55 and O75) with a percentage 11.1%, 22.2%, 33.3%, 11.1%, 11.1% and 11.1%, respectively.Identification of C .Perfringens isolates by using dermonecrotic reactions in albino guinea bigs revealed to present 21 toxigenic strains and 10 non- toxigenic strains from total 31 C. Perfringens strains. Toxigenic strains gave dermonecrotic reactions ofTyping of C. Perfringens isolates by using multiplex PCR that is more sensitive and specific protocol than sero-neutralization test. Our results reveald that 14 isolates had alpha toxin gene only and identified as C. perfringens type A, four isolates had The percentage of C. Perfringens type A, C, D and B according to total numbers .
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