Avian influenza (AI) is a highly contagious disease, able to spread in asusceptible population in a short period of time. Therefore, the promptidentification of an infected flock is crucial for control and eradication purposes.The present investigation was under taken to isolate and identify AIV fromchickens and ducks. A total of 160 samples were collected from chickens andducks. Embryonated SPF chicken eggs and Vero cells were used to isolate andpropagate the virus followed by virus identification by HI, IF and virusneutralization assays.The collected samples were inoculated on 10 day old embryonated SPFchicken eggs. The virus was isolated from 35/50 and 20/50 samples fromclinically infected and apparently healthy chickens with a percentage of 70%and 40%, respectively. In ducks, the virus was isolated from 21/30 samples and19/30 samples that collected from clinically infected and apparently healthyducks with a percentage of 70% and 63.3 %, respectively. No hemorrhagicembryos were detected in some samples that collected from diseased chickensand ducks.All collected samples were subjected to inoculation on Vero cells andexamined daily for detection of cytopathic effect. In chickens, the samples thatshowed CPE were 30/50 and 16/50 samples that collected from clinicallyinfected and apparently healthy chickens with a percentage of 60% and 32%,respectively. In ducks, AIV was isolated from 14/30 samples and 17/30 sampleswith a percentage of 46.6 %and 56.6 %, respectively.