Some studies on rift valley fever virus

Rift Valley Fever VIrus is an arthropod borne VIrus transmitted bymosquitoes that infect human and animals. RVFV induce almost 100% mortalityamong young animals and high rate of abortion in pregnant females. In humansinfection can vary from mild to very sever clinical picture, including temporaryand definitive blundness, encephalitis and hemorrhagic fever syndrome andhepatitis with a fatal outcome. The corner stone of virus control is eradication ofmosquitoes and vaccination and recently have control by antiviral drugs so inthis study we study effect of amantadine hydrochloride and acyclovir on RVFVreduction-or prevention in vitro (in BHK.-21 cell) and in vivo in (baby mice).In this study we evaluate the effect of amantadine hydrochloride toxicity onBHK.-21 cells and baby mice (5 - 15 gm) and found that amantadine has safeeffect on cell culture from 25 ug/ml to 500 ug/ml and have toxicity effect onBHK.-21 cell at 1000 ug/ml till 5000 ug/ml and not have toxic effect in mice till5000 ug/ml.. The effective dose of amantadine hydrochloride in vitro ranged from 100ug /ml to 500 ug/ml give reduction effect to infected BHK.-21 cell wheninoculated with or before inoculation with RVFV and have not effect afterinoculation of RVFV, but in mice (5 - 15 gm) has in vivo treatment use by highdose as dose of treat of 1000 ug/rnl to 5000 ug/rnl with or after or beforeinoculation with RVFV.To evaluate the effect of AB on RVFV by titration test and lab in tissueculture (BHK.-21 cells), 25 ug/ml and 50 ug/rnl dose not stop RVFV at treatbefore or after or with RVFV infection. But doses from 100 ug/rnl to 500 ug/mlstop RVFV at injection before or with RVFV infection but at treat after infectionnot stop virus and this measure by reduction rate and found the reduction is