| Abstract: |
Though the Arabian camel has been considered as one of the most popular domesticated animals in Arab world, little researches have been undertaken when compared to other animals especially in molecular biol-ogy. Knowing the genetic structure of a species DNA analysis technique via RAPD-PCR was found to be more powerful in detecting genetic variation in camels. The genetic diversity and relationships amongst the dromedary population are poorly documented. So this study were designed to evaluate the geneticBlood samples were collected form jugular vein of twenty five different individual camels from six subtypes belonging to three Egyptian camel types (Fellahi, Maghrabi and Mowalled) using 10 ml tubes containing EDTA as anticoagulant and transported directl50 Summary and conclusionmolecular biological grade. Then we take the resultant DNA after purification and making quantitation using GeneQuant II spectrophotometer.Protocol of RAPD-PCR was outlined according to Swanson et al., (2006). That includes three steps: denaturation, annealing and extension repeated to 40 cycles using six arbitary short oligon- ucleotide primers G-1, G-2, GC-10, GT-10, OP-08 and C-7. RAPD-PCAn effort has been made to determine genetic analysis for genetic differences in the local camel breeds, which have so far been differentiated only on the basis of phenotypic appearance, geographical location or on the tribal ownership. The data collectedOur study depends on selection of Egyptian camels on the basis of their DNA fingerprinting and find genetic similarity between them by using RAPD-PCR method. The RAPD profile of Egyptian camels breeds were analyzed. A total of 61 bands were amplified, of 51 Summary and conclusion(92%). The analysis of the electrophoretic patterns revealed a high polymorphism reach to 100% with primer: G-1, G-2, GC-10, GT-10 and 66.6% to 87.5% with primer: OP-08, C-7.Analyzing results of dendrogram show that with primer. G-1: Fellahi and Mowalled types closer but Maghrabi and Fellahi types are very apart from each other. Primer. G-2: Maghrabi and Fellahi types are very apart from each other. Primer. GC-10: Fellahi andOnly primer. OP-08 gives a close relationship between Maghrabi and Mowalled types.The probability of obtaining identical fingerprints was observed to be the lowest in primer GC-10 followed by OP-08, GT-10, G-2 and G-1. The primers GT-10 and OP-08 amplified a total of 17 and 16 bands respectively.There are three types of Egyptian camels distributed all over the country. Fellahi breed which is large in size white in color, Maghrabi breed which is medium and brown in color and the last breed is Mowalled which is dark brown. Sudani breed may be found
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