| Abstract: |
Sheep and goats are from the important agricultural animals in Egypt, Mycoplasma is one of the causitive agent of pleuropneumonia, mastitis, and affect on other organs due to septicemia in sheep and goats as it is the most serious epidemic disease causingIn this study, a total of 189 animals were included from which there were 116 sheep and 73 goats. A total of 610 samples collected from nasal swabs, conjunctival swabs, lung samples, Liver samples, spleen samples, tracheal and mediastinal lymph node samplThe numbers of collected samples from sheep were 369, from apparently healthy animals and 32 from diseased animals.The samples included 25 nasal swabs, 25 conjunctival swabs, 91 lung samples, 87 liver samples, 91 spleen samples and 50 tracheal and mediastinal L.N samples.As for goats, the numbers of collected samples were 34 samples from apparently healthy animals and 207 from diseased animals. The samples included 23 nasal swabs, 23 conjunctival swabs, 50 lung samples, 50 liver samples, 50 spleen samples, 45 tracheal andfrom sheep: A total of 286 Mycoplasma isolates were isolated with an incidence of (77%)from goats: A total of 200 Mycoplasma isolates were isolated with an incidence of (83%).All 486 isolates were subjected to digitonin sensitivity test to differentiate between Mycoplasma and Acheloplasma genera. All the recovered isolates in the present study were digitonin sensitive so they belonged to genus Mycoplasma except 31 samples wereAll 486 isolates, 286 from sheep and 200 from goats were subject to the following biochemical tests, glucose fermentation, arginine deamination and film and spot formation. The results revealed the presence of three distinct biochemical groups from both sGroup (1) which showed glucose negative and arginine positive and film and spot test negative were found in 37 isolates from sheep (3 from nasal swabs, 1 from conjunctival swab, 8 from lung samples, 10 from liver samples, 10 from spleen samples and 5 fromGroup (2) which showed glucose positive and arginine negative and film and spot test negative were found in 57 isolates from sheep (7 from nasal swabs, 6 from conjunctival swabs, 12 from lung samples,11 from liver samples,11 from spleen samples and 10 froGroup (3) which showed glucose negative and arginine negative and film and pot test positive were found in 174 isolates from sheep (1 from nasal swab, 4 from conjunctival swabs, 50 from lung samples, 46 from liver samples, 48 from spleen samples and 25 frSerotyping of isolates for each biochemical group in both sheep and goats against available antisera revealed the identification of:1- M. arginini by a percentage 13.8% in sheep and 55.6% in goats.2- M. ovipneumoniae by a percentage of 21.26% in sheep and 20.32% in goats.3- M. agalactiae by a percentage of 64.92% in sheep and 24% in goats.PCR technique was found to be much faster than conventional microbiological techniques for isolation and identification and results could be obtained within five hours. In the present study six field isolates of M. agalactiae were tested by PCR, the the r
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