Comparison of Polymerase Chain Reaction and Conventional Methods for Diagnosis of Avian Salmonellae

Foodborne infections in man including salmonellosis increased as a whole in the last years. The identification of infected commercial poultry flocks has become pivotal component of efforts to reduce the incidence of egg-associated transmission of S. enterof peR technique in diagnosis of avian salmonellae in comparison with the conventional methods. In this concern our study was conducted to achieve the following points: Isolation of Salmonella species from layers by conventional bacteriological methods.Biochemical identification of salmonella isolates. Serotyping of the biochemically identified salmonellae,4) Determination of the genotypic characteristics using PCk including the presence of virulence inv A genes at different isolated serovars.Analysis of the whole cell protein of different isolated serovarsby sodium dodecyl sulphate polyacrylamide gel electrophoresis(SDS-PAGE). In the present study, a total of 88 layer flocks were surveyed for lcreening the flocks’ state concerning salmonella infection from May,