Bacteriological and immunological studies on probiotics

During the last two decades , a great attention was paid towards the use of probiotics as food supplement not only supporting the growth of normal bacterial intestinal flora but also having a beneficial effect onthe feed conversion ratio , so this study was planned as a trial toinvestigate the following items :1- Isolation and identification of the bacterial constituents of someprobiotics .2- Determination of phagocytic activity of chicks fed on feed supplemented with probiotics and make a comparison between treated .and untreated control group of chicks.3- Performing the challenge test by using E.coli 078:K80 forexperimental infection of both treated and untreated control groups of chicks.The obtained data proved that:1- A statistical significant differences in the phagocytic % and phagocytic index between chicks of probiotic treated and untreated groups2- The increase in phagocytic % in Group B (Treated with 1. acidophilus)71.50%, 14%, 7.5% and 6.35% was significant than the other groupsand the untreated control group J (Negative control not treaed withprobiotic bacteria) 33.750/0, 7.50/0, 7.5% and 3.075% followed by GroupD (Treated with E. faecium) 55.40%,13%, 10% , and 5.5% Group A(Treated with B.subtilis natto) 47.50%, 11.5%, 7.5% and 2.5% and finallyGroup C (Treated with 1. acidophilus, casei, E. faecium and