| Abstract: |
The buffaloes are considered one of the most animals reared in Egyptian village which may be attributed to its resistance to diseases, its good quality meet and milk and its high conversion rate that make it more economic than others. Natural service stilThe semen was collected twice weekly using artificial vagina in each one 2 ejaculates was collected by 10 minute interval.The semen was evaluated including the volume, color, viscosity, pH, mass and individual motility, % of live and dead, % of sperm abnormalities, sperm cell concentration per mm3, metabolic activity, resistance of sperm cell to dilution and cold shock and lThe semen was gradually diluted, cooled and preserved at refrigerator (5oC). Moreover, evaluation of preserved semen was also carried out including the percentages of motile sperms, estimation of viability indices, the livability percentages and the acros1- By using TRIS egg yolk diluent, filtrated TRIS egg yolk, once centrifugated TRIS egg yolk and twice centrifugated TRIS egg yolk to obtain 4 different diluents in experiment no 1. The results revealed that once centrifugated TRIS egg yolk and twice cent2- By using milk diluent, milk egg yolk diluent, milk egg yolk once centrifugated diluent, milk egg yolk filtrated diluent, milk once centrifugated diluent, milk once centrifugated egg yolk diluent, milk filtrated diluent and milk filtrated egg yolk dilue3- By using different ratios of the best diluents (once centrifugated TRIS egg yolk and once centrifugated milk egg yolk diluents) 0:100, 10:90, 20:80, 30:70, 40:60, 50:50, 60:40, 70:30, 80:20, 90:10 and 100:0 to obtain 11 different diluents. The results from these results we can conclude that centrifugation of the egg yolk diluents well increase the preservation capacity of the diluents this may be due to the concentration of the main protective material of the egg yolk and removal of some harmful materi
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