Immuno virological studies on neonatal calf diarrhea virus infection in dairy farms in Egypt

Neonatal calf diarrhea constitutes a serious problem to rearing of bothlry and beef cattle all over the world. BRV and BCoV are considered the most.portant pathogens, causing serious economic losses to dairy and beef industry occur mostly between 5 and 10 days of age and virtually always under 3 eks of age. Therefore, the present study was conducted to isolate and identify ~BRV and/or BCoV in calves:gnancy by killed adjuvated trivalent (BRV, BCV and K99 E.coli) vaccine.I In the present study, a total of300 (230 calves and 70 buffalo calves) rectal ab samples were collected from vaccinated or non vaccinated animals, and m clinically affected and contact animals. All collected samples were used in isolation attempts on MDples out of 300 tested samples (16.3%)). (There were 18 and 31 samples out 142 vaccinated (12.6%) and 158 non vaccinated herd’s samples (19.6%)~ctively. The viruses were isolated from 31 and 18 out of 119 diseased.0%) and 181 contact (9.9%) calves samples respectively. There were 10 out16 native calf’s samples (21.70/0) and 27 out of 184 imported calf’s samples,6%). The virus isolates (49 samples) were identified by VNT. BRV was detected 19 out of 49 tested samples (38.7%), there were 14 out of 37 calves (37.8%) 5 out of 12 buffalo calves (41.6%). BCo V was detected in 9 out of 49 tested ~Ies (18.3%), there wer