Application of Some Biotechnological Methods For The Diagnosis Of Mycoplasmal And Bacterial Mastitis In Cows and Buffaloes

Mastitis is of great economic importance in dairy animals resultingm reduction of milk yield or even destruction of mammary gland. Determination of the most causative agents of mastitis is one of our targetin this study either bacterial and or mycoplasmal causes. 235 animals were incorporated in this study including 157 cows and 78 buffaloes with an overall 695 quarters showing signs of mastitis (461 quarters from cows and 239 quarters from buffaloes). Oversigns in two quarters, 90 showed signs in three quarters and 80 showedsigns in four quarters with overall 235 animals including 695 quarters.High prevalence of mastitis was observed in quarters harboring bacterial infection in winter season in both cows and buffaloes with an incidence of 4l.29% and 43.47% respectively followed by autumn season (32.33% and 22.82%) ; spring (16.41 % and 20.650/0autumn season (30.0% and 23.33%); spring (20.0% and 16.660/0). Lastly,the lowest incidence was recorded in summer with an incidence of 8.75~/0and 13.33. Mixed infection was also found in this study and it is observedthat the highest prevalence was recorded in winter in cows and buffaloes (6l.5% and 47.82%) followed by autumn (38.5% and 39.13%) meanwhilein spring season infection was not found and in summer in cowsmeanwhile in buffaloes the incidence of mixed infection was 13.04%.