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Microchip analysis of plant glucosinolates
Faculty
Pharmacy
Year:
2008
Type of Publication:
Article
Pages:
2280-2287
Authors:
Kaji, Noritada, Tokeshi, Manabu, Baba, Yoshinobu, Terasaka, Kazuyoshi, Mizukami, Hajime, Fouad, Maged, Jabasini, Mohammad
DOI:
10.1002/elps.200700635
Journal:
ELECTROPHORESIS WILEY-BLACKWELL
Volume:
29
Research Area:
Biochemistry \& Molecular Biology; Chemistry
ISSN
ISI:000257011600006
Keywords :
glucosinolates, microchip capillary electrophoresis, self-organizing map, xanthene dyes
Abstract:
We describe a new and selective analytical method for the separation and quantitation of plant glucosinolates. The new method, which utilizes microchip CE (mu-CE) with fluorescence detection, circumvents the multistep procedures characteristic of conventional methods. Glucosinolates form charge transfer complexes with the xanthene dyes phloxine-B and eosin-B. The glucosinotates-phloxine-B complex cannot be excited at 470 nm. Thus, the decrease in peak intensity of phloxine-B after complex formation is used to quantitatively measure total glucosinolates in Arabidopsis thaliana seeds. For qualitative analysis, complex formation with eosin-B is used. The sensitivity of eosin-B detection at excitation/emission 470 nm/540 nm was low However, sensitivity increased following complex formation with sinigrin (>= 3 mu g/mL). A batch-learning, self-organizing map was applied to visualize and organize analytical data into 2-D matrix with similar and related data clustered together or near each other. This organized matrix was used to optimize electrophoretic conditions for the analysis. This study suggests potential applications of mu-CE in plant metabolomics analyses without use of labeling fluorophores.
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