Studies On Intestinal Flora Changes In Native Hens During Natural Brooding And Its Defensive Importance To The Progeny

This work aimed to study the changes in the intestinal microflora inbroody and non broody balady hens, as well as in naturally and artificiallyhatched balady chicks. Moreover evaluation of protection ability of Normalintestinal microflora preparation (NIMP) and competitive exclusionproduct (CE) Broilact against experimental infection with Salmonellatyphimurium and Eimeria tenella in balady chicks.1 – Experiment IAmong the normal intestinal microflora; Escherichia coli, Enterococci,Lactobacilli and Clostridium perfringens were successfully isolated fromfaeces of all examined eleven month old broody and non broody hens atdifferent periods. The total anaerobic count was higher in broody baladyhens at 5th and 10th day of brooding (8.61 ± 0.25 ) and (8.72 ± 0.22)respectively than in the non broody (8.25 ± 0.40) which were synchronizedwith a decline in pH of broody hens at 5th and 10th day of brooding 6.07 ±0.05 and 5.94 ± 0.06 respectively. where a significant rise in prolactinhormone level was detected in broody hens (6.31 ± 0.12) pg/ml serum ifcompared to non broody hen (292 ± 0.21) pg/ml serum.2 – Experiment IIThe total anaerobic count of caecal contents in the naturally hatchedchicks up to 21st day of age were significantly higher than in artificiallyhatched ones.