The present study was conducted to investigate the different types of chromosomal aberrations of chicken embryos as well as newly hatched chick and their relationship with fertility of domestic chicken. Three breeds were used in this study a sample of 737were examined by using the reverse real time polymerase chainreaction to the avian influenza virus (Real time peR) types (M: H5:H9: H7: NI) under different thermal conditions using QIAGEN Kits and a STRATEGEN apparatus in the National Laboratory for