In this study , we used fourteen healthy New Zealand rabbits which were obtained from the faculty of Vet. Medicin farm, Zagzig , University.The individual body weight ranged from 3 ± 0.025 kg . The age of the begining of the experiment about 5 months ± few days. They were all sexually mature and non pregnant, and were supplied on balanced ration. The rabbits were divided into 3 groups. 1 st g2 nd group contain 5 females (as control group) 3 rd group contain 2 males for mating and breeding.All rabbits were free from any pathogen and parasite. We collected blood samples to obtain serum to determine cholesterol triacylglyceride, lipoproteins (HDL,LDL,VLDL) and phospholipids. And some important trace elements such as iron, copper, zinc andselenium at day (0) before mating, at 7th day of pregnancy, at 14th dayof pregnancy, at 21st day of pregnancy, 28th day of pregnancy, and after parturition at32nd and 37th day.We observed that: Cholesterol : showed significant decreased at the day 14 th , 21 st and 28th of pregnancy ,the levels returned near to the normal values at the day 32 nd after parturition.