| Abstract: |
The present study was carried out to investigate brucellosis on 219cows, 50 buffaloes, 105 sheep, 16 goats and 500 of one humped camels(camelus drornedarius) of different ages and sex and at a various sourcesat Sharkia governorate to clarify the prevalence of the disease and to findthe most reliable diagnostic method for burcellosis which can be adaptedfor eradication of the disease under regional condition in those area inEgypt.In this investigation serological examination of 219 cows, 50buffaloes, 105 sheep and 16 goats using Rose Bengal plate (RBPT),Buffered acidified plate antigen (BAP A), tube agglutination (TAT),complement fixation (CFT) and Enzyme linked immunosorbent assay(ELISA) tests. It was found that RBPT showed positive reactors 8(3.65%) in cows, 2 (4.00%) in buffaloes, 4 (3.80%) in sheep and 1(6.25%) in goats, also BAPA test showed positive reactors 8 (3.65%) incows, 2 (4.00%) in buffaloes, 4 (3.80%) in sheep and 1 (6.25%) in goats,TAT showed 8 (3.65%) in cows, 2 (4.00%) in buffaloes, 4 (3.80%) insheep and 1 (6.25%) in goats, also CFT gave positive reactors 8 (3.65%)in cows, 2 (4.00%) in buffaloes, 4 in sheep and 1 (6.25%) in goatsalso ELLS,, A showed positive reactors 7 (3.20%) in COW<3, 2(4.00%) in fuffaloes, 4 (3.80%) in sheep and 1 (6.25%) in goats.
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