| Abstract: |
50 random processed cheese samples were collected from differentshops in Sharkia govemorate .A survey was under taken to determine theincidence of moulds and their toxins.All tested samples proved to be contaminated with mould. Themean mould count / g. was 1.16 x 10 ~ ±- 0.02 x 10 ~ . Aspergillus,Cladosporium, Fusarium, Geotrichum, Mucor, Penicillium, Rhizopusand Syncephalastrum could be isolated from examined processed cheesesample with a varying percentages ranged from 100/0 to 1 00~1o. Out of581 isolated mould strains obtained from overall examined samples were264 (45.45 (Yo) Apergillus, 181 ( 31.15 0/0) Penicillium, 86 (14.80 %)Cladosporium, 19 ( 3.27 % ) Rhizopus, 10 ( 1.72 % ) Mucor, 9 ( 1.55 % ). Geotrichum, 7 ( 1.20 0/0) Syncephalastrum and 5 ( 0.86 <?’o ) Fusarium.Moreover, isolated Aspergillus Species were futher identified intoA. flavus, A. niger and A. terreus and could be found at varymg’. percentages ranged from 16 % to 84 % of the examined samples .Out of 264 isolated Aspergillus strains obtained from all examinedsamples were 181 (68.560/0)A.flavlls, 72(27.270/o)A. niger and 11 (4.17 0/0)A.terreus.A total of 42 A. flavus strains were screened for their ability toproduce atlatoxins . TLC analyses showed that 17 ( 40.47 %) Aflavusstrains were toxigenic. Atlatoxin Bi, B2, Gl and G2 produced by 29.41 %,17.64 0/0, 35.29 % and 17.64 % of toxic strains isolated from examinedsamples. The range of estimated total atlatoxins ( 4 types) were 1.25 -8.62 fig. / I it. of culture filtrate.
|
|
|