Epidemiological studies on some zoonotic bacteria of food borne diseases

Epidemiological studies of some zoonotic food borne bacteria invarious wastewater types, cattle slurry and poultry manure. One hundredand sixty seven wastewater samples were collected from 3 differentwastewater treatment units in Germany. Eighty five group samples werecollected from different regions in Germany, 27 samples were fromgroup cattle slurry, 27 were from herd swine slurry and 31 samples fromfresh poultry farm’s manure.Different bacteriological tests were conducted to determinequalitative and quantitative detection of Salmonella species andCampylobacter species. Serotyping, phage typing and antibiotyping hadbeen carried out for Salmonella isolates. The isolate~ Campylobacterspecies are confirmed by agglutination test. The samples were examinedfor detection of EPEC 0: 157, 0:26 and 0: 111 using immunomagneticseparation and HCI treatment methods. The isolates were confirmedserologically. Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR fingerprinting had been conducted for S. enteritidis, S. typhimuriumand Cjejuni isolates from different sources. Molecular-biological studieshad been carried out for detection of stxi, stx2, eaeA andEnterohemorrhagic E. coli hlyA genes in E. coli isolated from cattle andswine slurry using Multiplex PCR Assay.The obtained results were summarized as follows :1- The mean coliforms count in mixed out put wastewater were found8.12 x102 cfu/g