| Abstract: |
This study was done at Marriout Research Station that belongs toDRC (Desert Research Centre). 95 rabbits were used, equally classifiedinto 5 groups (gTOUp 1,2,3,4 and control). Group 1 and 2 subjected to behoused under hear stress. Groups 3, 4 ,lIld control housed under normaltemperature. Two feeds were used, Fresh commercial feed pellets usedfor feeding groups 1, 4 and control and stored commercial feed pellets for6 months under desert conditions and contaminated with aflatoxin (1532ppb), were used for feeding of group 2 under heat stress and group 3under normal temperature. All groups were supplied with clean tab waterand fed ad-libitum while groups 4 were fed restricted to 75% of theaverage feed intake. Whole blood, plasma, and tissue samples were takenat day 1, 3, 7, 14 and 21 of the experimental periods. Whole bloodsamples were used for determination of glutathione peroxidase activitiesand superoxide dismutase activities. Plasma samples were used fordetermination of malondialdhyde levels (MDA), cholesterol,triglycerides, plasma total protein, total lipids, fatty acid composition,calcium and inorganic phosphorus levels. Liver homogenates were usedfor determination of malondialdhyde level, total liver protein andphospholipids content.The results showed decreased activities of glutathione peroxidaseand Superoxide dismutase in all groups at day 7, 14 and 21 of theexperimental periods, that results denote to the inability of the antioxidant defense system to remove the excess production of free radicals.Increased levels of (MDA) in serum and liver tissues in all groups, whichrefers to the increased rate of lipid peroxidation.
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