Histop Athological and Histochemical Changes Induced Through Transplacental Transmission of Chemical Carcinogen in Mice

One hundred female mice were used in this experiment to study thepathological changes induced by methylnitrosourea among different organs of dams mice and their offspriugs with special emphasis on the incidence and types of the different developed neoplasms (20 male mice were used only for mating).The experimental animals were divided into 5 groups:Group I: (20 pregnant female mice), each animal was injected with3 doses (50 mg/kg b.w. from methyl nitrosourea) (2 mg/mouse) on 7,14and 18 days from beginning of pregnancyThere were (50%) mortalities in the first month after injection.After 120 and 150 days post-treatment respectively keratoacanthoma of skin (60%) and Adenocarcinoma of sebaceous gland (20%) were developed in injected mice at the site of injection.The liver showed congestion of blood vessels, hypertrophy of Kupffer cells and some of hepatic cells showed precarcinogenic changes represented by large hyperchromatic nuclei with mitotic activities, the portal area revealed hyperplasia of the bile ducts.some renal tubules. The brain showed degeneration of the nerve cells represented by swelled deep basophilic nuclei satellitosis and neuronophagia and encephalomalacia were also detected. The spleen