Application of some molecular biology techniques in breeding and reproduction of farm animals

Molecular biology is considered an ’irnprotant tool to beapplied in breeding and reproduction of farm animals.AThe present work was carried out for the parentage identi ficationand inbreeding in horse as well as assessment of inbreeding in sheep.The Inaterials of this wor)\. were:a) Horse (include 9 horses, 4 femael and 5 male). These animals wereraised at EI-Zahraa farm, Cairo.b) Sheep (include 12 ewes) which raised in the farm of the faculty ofVet.Medicine, Zagazig. These animals were grouped into fourgroups according to age.Blood samples from sheep as well as from horse were collected onEDT A as anticoagulant. DNA was extracted from blood then subjected todigestion with restriction enzymes, electrophoresis, southerntransfer,hybridization with labelled probes and immunological detectionof the sites of hybridization.1- Parentage identification in horse: was done byb) Hinf J/(OAT)18 enzyme / probe combination: The resulted band patternranged from 23 to 3 kb size range. Some individuals showed paternityrelationships (values 2 0.5). While other individuals showed lowervlaues. Exclusion probabilites ranged from 8.29 x 10-8 - 9.1 x 10-13• Group /11: (subgp. A and B) orally received combination of (0.1 mgestrogen + 2.5 mg progesterone) and (0.05 mg estrogen + 1.5 mg progesterone )/rat respectively.Group IV: served as untreated controls.The administration of above mentioned drugs to the animals were continued six days weekly until the time of death or sacrificed (16, 24 and 36 weeks).Clinical signs and pathological findings were recorded: Group (1)which received estrogen alone showed emaciation, decrease in body weightin most rats beside loss of hair particularly on the pelvic region in rats of subgp, (A,B). The uterus in most rats was slightly enlarged and contained 2- 3 ml of serous fluid. It was measured (2-3 times of its normal size with