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some biochemical studies on the effect of some antimetabolites on blood constituents and tissues of mice
Faculty
Veterinary Medicine
Year:
1998
Type of Publication:
Theses
Pages:
136
Authors:
Victoria Ramzy Habib
BibID
10343469
Keywords :
Veterinary Clinical Biochemistry
Abstract:
- The effect of antimetabolites on some biochemical parameters inblood constituents and liver glycogen content of female mice wasinvestigated.- For performing the present study, 210 white female mice wereused.They were classified into two groups as follows:I. The first group: non tumor bearing-mice (NTBM). It comprised 30female mice, and was considered as control group.11. The second group: tumor-bearing mice (TBM). It comprised 180female mice classified into three subgroups:2. It consisted of 60 TBM, considered as control.3. It included 60 TBM, administered with MTX.4. It contained 60 TBM, administered with 5-Flu.- Blood samples were collected from 3 female mice after being killedby decapitation, in clean tube containing anticoagulant and processeddirectly for the estimation of glucose, lactate, insulin, ketone bodies,free fatty acid, triacylglycerol, cholesterol, total protein and albumin.- Liver samples were collected and processed directly for thedetermination of glycogen content.- The obtained results could be summarized as follows:* Glucose:Plasma glucose content was significantly decreased in TBM, whileafter administration of antimetabolites it became slightly increased.* Lactate:The concentration of plasma lactate showed a significantly increasein TBM, followed by a decrease after administration of MTX or 5-Flu.
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