Evaluation of Different Laboratory Techniqres for The Diagnosis of Clostridium Chauvoei In Guinea-Pigs With Emphasis on The Immunologic Response

Clostridium chauvoei is a strictly anaerobic organism which causesblackleg disease that mainly affects cattle and sheep. In Egypt the disease is successfully controlled by using alum precipitated Cl.chauyoei vaccine.The present work aimed to evaluate the different laboratory techniquesused in diagnosis of Cl.chauvoei infection including thebacteriological, hematological, serum biochemical analysis and pathological studies onlaboratory animal ”guinea-pigs”. Also, to investigate the immune response ofblackleg alum precipitated vaccine in laboratory animals by immune- serological, hematological, serum biochemistry and pathological means.The current work was divided into two experiments. The first experimentwas of one hundred and twenty female guinea-pigs were divided into twogroups. The first group including 95 female guinea-pigs vaccinated with alumprecipitated C1.chauvoei vaccine in two successive doses, the first dose was2ml sic and the second dose was 1ml sic with 21 days apart. Weekly post-vaccination with the first dose we sacrificed 5 guinea-pigs till 5th week post- vaccination. The remainent 70 guinea-pigs divided into two equal subgroups, vaccinated subgroup CA) and challenged subgroup (B) which inoculated with 0.5 ml. of Cl.chauvoei 18