Experimental pathologic studies on bilharzial cirrhosis in mice

Two hundred and sixty albino mice were used to study the possibility of using either single or double doses of ETT for vaccination or treatment of schistosomiasis. They were divied into 13 equal gps (4 gps. for each of the experimental control, vaccinatio(40 or 20). The 4 vaccinated gps. were given 60 ETT (as a single or 2doses) followed by 40 or 20 SMC. The 4 treated gps were first infestedwith 40 or 20 SMC and 60 day later were given the ETT as a single dose(60) or double doses (each of 30). Another gps. was kept as control. Miceof experimental control and vaccinated groups were sacrificed 60, 90 and120 day PI or PC; respectively. While those of treated groups weresacrificed 30, 60 and 90 day PC. Specimens from the liver were preparedfor histopathology and electron microscopy.The criteria used for evaluation of the vaccination and treatment ofSchistosomiasis included the mortality rate, the number of schistosomalworm recovery, the number and size of egg-nodules per microscopicfield (obj. x 10) beside the stages and structure of egg-nodules andretrogressive changes and necroses of the hepatic parenchyma.The mortalities of the control groups were 25% (60 ETT), 30% (30+ 30 ETT), 80% (40 SMC) and 80% (20 SMC), respectively. Thevaccinatd groups showed 30%, 35%, 25% and 25% mortalities and thetreated groups showed 60%, 60% and 65% mortalities, respectively.