Comparative Clinico Pathological Studies on Clostridium Perfringens Exotoxins In Guinea Pigs

cL perfringens constitute a great problem for animals as it can causesudden death in addition to severe renal, hepatic and gastrointestinal damage with marked hematological changes. This study inculded two experiments. The first one comparised 66 animals, 42 of them were subdivided into 3 study groups, where the animals wedifferent doses of exotoxins produced by CL perfringens type ”D’’. For eachstudy group a control group (each formed of 8 animals) was injected alsowith the same volume of physiological saline instead of toxin. Group 1consisted of 16 guinea pigs injected with 112 M.L.D as single injection.second group was injected with 114 M.L.D as single injection, the animals in the two t,lTOUpS were sacrificed 48 hrs. after injection. the 3rd group injected with liS. M.L.D twice weekly for three weeks, and the animals weresacrificed at the end of experiment. The second experiment comprised 76animals, 52 of them were subdivided into 4 study t,lTOUpS, the animals wereinjected by intraperitoneal route with four different doses of exotoxinsproduced by CL. perfringenstype” A”. For each study group a control !:,lTOUp(each consisting of eight animals). Group IV consisted of 16 guinea pigseach of them was injected intraperitoneally with 112 M.L.D as single injection, !:,TfOUp V consisted of 16 animals. each of them was injected with 1/4 M.L.D as single injection and the animals in both blTOUP were sacrificed48 hrs. after injection. Group VI consisted of 10 guinea pigs each of them was injected with 1/8 M.L.D, all the animals in this group died within six days from the first injection, blTOUP VII consisted of 10 animals, each of them was injected intrapritone