| Abstract: |
Three hundred and fifty-five albino mice were used to study the ability of TVL to be used as a vaccine or treatment against schistosomiasis through the role of eosinop’uls . These mice were divided into 10 gps.Five of them were experimental control gps.ccinfested with 40 SMC (gp.l ), 1,000 ETY (gp.2) ,1,000 + 1,000 ETY (gp.3), double doses of 40 mg/kg .B.wt .praziquantel (gpA) anduninfested control (gp.5)” .Other two groups (6 and 7) were vaccinatedwith 1,000 (gp.6) or 1,000 ETV (gp.7) and challenged with 40 SM~.Groups (8,9 and 10) represented the treated gps.”infested with 40 SMCand treated with 1,000 ETY (gp.8), double doses of 40 mg/kg .B.wt .of praziquantel (gp.9) or praziquantel followed by 1,0(gp.lO)” .The schedule of sacrifice for the experimental control groupswas 60 ,90 and 120 day PI (gp.l), 7,14,21 and 40 day PI (gp.2) or PC(gp.3),3,7,21 and 40 dayPA(gpA) and 3,7,14,21,30,40,60,90 and 120day (gp.5) .The vaccinated gps (6 and 7) were sacrifced 60,90,and 120day PY; . meanwhile the treated gps. (8,9 and 10) were sacrificed 30,60and 90 day PT. Specimens were collected from the liver, intestines,pancreas, spleen, lungs, kidnys, heart and brain. Five micron thickparaffin sections were prepared and stained for histopathologicexamination. Blood samples were collected for clinicopathologicstudies. The criteria used for evaluation of the vaccination and treatment of schistosomiasis, included the mortality rate, the number of bilharzial worm recovery,the number and size of egg-nodules per microscopicfield(l Oxl 0), the stages and structure of egg-nodules,the retrogressive changes and necroses of the hepatic parenchyma beside theclinicopathologic findings.The mortalities were 94%(gp.l), 20%(gp.2),8%(gp.3), O%(gpsAand 5), 24%(gp.6) and 16% (gp.7),56% (gp.8) 34% (gp.9) and 42 %(gp.10).Mice,infested with 40 SMC, showed focal replacement of thehepatic parenchymawith 7-12 egg-nodules (stages 1,2 and 3 each withJ-10 eggs)per microscopic field (lOxl0).Coagulative necroses withapoptosis, severe congestion, hemorrhages,thrombosis and infarcts were encountered in the liver beside adult schistosomes in the portalcinsand bilharzial pigments ip”ide the kupffer cells . nd in the macrophages
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