Biological studies on trichomoniasis in birds

The present study was carried out to explore the of biological studiesstatus of Trichomoniasis in pigeons. The representative samples of pigeons(squabs and adults) were collected from different localities in SharkiaProvince; [Abu Hammad, Al-Gaafaria, El-Asidia, Zagazig, (Shiba, Bahnaby,Bany Amer) and Helmia], from December 1990 to November, 1991.It was found that 225 pigeons out of 386 were positive for Tichomonas gallinae with percentage of 58.3%. The percentage of infection among males, females and squabs were 42.7%, 55.5% and 68.5% respectively.Seasonally, the percentage of infection was highest in spring to be(82.2%) in squabs followed by females 72.2% then in males 52.4%. Inwinter the infection was 66.7% in squabs followed by females 48.3% then38.9% in males. In summer the percentage of infection was 64.5, 53.6 and 48.7 in squabs, females and males respectively. In autumn the infection with T gallinae in squabs females and males was 57.l%, 48.60/0 and 43.80/0respectively.In the present study the parasite was cultivated on different media.The glucose-broth serum medium gave the highest population density of theparasites (600 parasites/ml). Simplified trypticase serum medium yield 450 parasites/ml and 300 parasites/ml in beef extract-glucose pepton medium after 48 h. post-inoculation.The parasite was also cultivated in chicken embryo via three differentrouts {yolk sac, allantoic cavity and chorio-allantoic membrane (C.A.M.)}•at 5, 9 and 11 days respectively. After 48 H.P.I. the maximum. growth of theparasite was on the (C.A.M.) in living embryos and their number ranged