Effect of a local Isolate of Hemorrhagic enteritis virus of Turkey on the Immune System and Trials for preparation of vaccine

The present work studied the adverse effects of a local isolate ofhaemorrhagic enteritis virus (HEV I) and preparation of a vaccine from thelocal isolate and evaluation of its potency as compared with an imported,commercially available live vaccine.In one experiment, the adverse effects of HEV 1 were studied bydetermination of the heterophil function, total leucocytic count (TLC),differential leucocytic count (DLC), total serum proteins, body weight gain,spleen index and immune response to Newcastle disease (ND) and fowlpox live virus vaccines in 8 groups of 52 days old turkey poults. Resultsachieved are summarized in the following: 1- Measurement of the heterophil percentage and phagocytic activity in heparinized blood stimulated with latex and unstimulated from 52 days old turkey poults infected orally with 103 median turkey infective dose Differences in both values between infected and uninfected groups wereinsignificant by day 21 P.I.2- The differential leucocytic count showed significant lymphyopenia onday 3 and 7 P.1 in groups infected with HEV I. Heterophil percent in allgroups ofpoults infected with HEV1 was higher on day 3 and 7 P.1 thanuninfected groups. Esenophils, basophils, and monocytes percent werenot significantly changed in both HEVI-infected and uninfected poults.3- The serum total protein levels were significantly lower at the 3rd, ih and14th day P.1 only in groups infected with HEV1 than in uninfected