Clinico-Pathological Studies on the Aminoglycosides Antibiotics Toxicosis In guinea Pig

The aminoglycoside group contains several members of antibiotics.In this work we used streptomycin, kanamycin and gentamicin becausethese three drugs are used on large scale in the veterinary and humanmedicine for treatment of diseases caused by Gram negative bacteria,and human tuberculosis (T.B).Toxicity is the major limitation to the use of these antibiotics byinjection, specially in the treatment of chronic diseases and human T.B.which take relatively long periods of therapy. The aim of this work is to study the changes in blood cytology,kidney tissue and kidney function at various durations of drugadministration, by therapeutic doses of these drugs, in guinea pigs. Thereversion of such changes were tested after the end of treatment courses.Ninety healthy adult male guinea pigs were used in this work,divided into 3 equal experiments, each one was subsequently subdividedinto 6 equal groups. The animal of experiments 1,2 and 3 were given,s.c. single daily therapeutic dose 155 mg streptomycin, 155 mg kanamycin and 16.28 mg gentamicin/kg.b.w. respectively. Groups 1,2,3 and 4 were given the drug for 7, 14,21 and 28 days respectively. Group 5 was given the drug for 28 days followed by 21 daysnormal saline) per animal. The blood and urine samples. were collectingduring 24 hours after the final dose from the animals of first four