Toxicological Studies on Eransplacental Transmission of Aflatoxin In Rat

Production of aflatoxin, this was carried out bycultivation of Aspergillus flavus (NRRL 3357) on two media(yeast extract sucrose medium ”YES” and sucrose low saltmedium ”SL”). The NRRL 3357 strain produce only aflatoxinBl, which extracted from media by chloroform, separated onTLC and estimated by HPLC. The sucrose low salt medium (SL)found to be suitable for growth and aflatoxin productionthan yeast extract sucrose medium (YES).The main clinical signs observed were vaginal blooddischarge and abortion in some cases. Dams did not preparedthe nest for parturition. Cannibalism by mother for someoffsprings especially stillbirth and some alive offsprings,was observed immediately after birth.The main postmortum findings were congestion ofmother’s liver, enlarged spleen and brown coloured placentawith some areas of necrosis. Congestion of liver of thefoet i .Developmental toxicity were a decrease in body weightof the offsprings especially at a dose level 1.8 mg/kg B.W in eithertwo periods but this decrease was more prominant in the latestage of pregnancy. Resorbed foeti and failure of conception