Experimental candidosis in mice

Two hnundred and fifty five apparently healthy mice ranged from 19 to 21 gm weight and proved to be free from candidosis were divided into two groups the first included sixty mice usedfor experiment induction of infection with 106 and 108 cells of Candida albicans by intravenousintraperitoneal, intracerebral and intranasal routes. The second group contained one hundred and ninty five mice were classified into two categories. The fristcategory cantained sixty mice used to study the toxicity of heatkilled cells of Candida albicans.The second category comprisedone hundred and thirty five mice used to study the effect of toxic extracts which were obtained from whole cells and cell walls of Candida albicans by hot phenol extraction. The obtained results could be Summarized as follow:1- Induction of experimental infection with candida albicans by various routesa- Mortality rate:-No deaths noticed in mice which injected with 1x106 cells byintravenous intraperitoneal and intranasal routes but theinoculation of mice with 1x106 cells intracerebrally produced fewmortatlites (40%), while the inoculation of mice with 1x108cellsby intravenous, intraperitoneal and intracerebral routes wereinduced mortality about 80% ,60% and 100% respectively theIntranasal route failed to induce of any deaths.