Role of gonadotrophin binding proteins in regulating reproductive performace of cows & buffaloes

In the present study it was deciced to investig8te the possible role played by the gonadotrophin bindingproteins (non steroidal serum albumin) in regulating the synthesis, secretion and transport of gonadotro- phins during the estrous cycle in cows and buffaloes.Blood samples were obtained from twenty Friesian cows.The animals were classified into 5 groups of 4- CO\~IS each at 0 time (estrus), 1 day (metestrus), 15 deys(diestrus) and 19-20 days postestrus (proestrus), and smooth inactive ovaries.Samples of blood from buffaloes during proestrus, estrus , metestrus, diestrus, and smooth inactive ovaries,were obtained from Cairo slaughterhouse. In these animals,the ovaries were taken and examined ~todetermine the phase of the cycle according to the size 9f G~aafian follicles and corpus. luteum.Serum albumin was extracted from serum samples by the alcohol method from which steroids were extracted by acetone-ether mixtures.Buffalo~FSH and LH were prepared and rabbits antisera to them were prepared to be used for immunoassay ofbuffalo and cow gonadotr6phins and detection of their