The present work was carried out on 16 ossemi ewesreared on the farm of Faculty of Veterinary MedicineZagazig University.The ewes were 2-4 years old and their weightsranged from 40-60 Kg. All of them were kept under thesame method of management and k2pt under good hygeinicThe animals were divided into [our goups ns thefollowing:Included 4 ewes served as control and fed on basaldiet coposed of commercial concentrate mixture having16% crude protein, hay and rice straw ad lib.Contains 4 ewes received basal diet raised to 22%crude protein in commercial concentrate mixture usingsoya been meal. isolated from subclinical mastitis in dairy Fresian cows with an incidenceof 13.2% and 10.9% respectively. While Strept. faecalis, Staph. aureus,Staph. xylosus, Staph. epidermidis and Pseudomonus aeuroginosa couldbe detected in 3.20/0, 3.2%, 2.7%,1.4% and 0.9% of samples respectively.