Preservation of cold and warm water fish gametes

The expert ments were performed on both cold wet er fish (Paintll)w trout, Brown trout and Grayling) and werrn water f ish (Cornrnon Carp).FOr each f sperm arnj eggs, Hie short tenn preservett on (chilling storage and cryopreservation were doneConcerni nq semen preservet ion, short term storeqe of undil uted sernen of Hie common carp caul d be preserved in an oxuqen etmospher at 2.2-3.7°C for 12 da!ds ”,’’.,1 it 11 Hie less of 23%of its rnot i1 itu. I’”leanwl-,i 1 e, Rai nbow trout, Bro’wn trout andGreul i nq sernen V’las cruopreserved usi nq diff erent dil uti onrnedi B. Sperrn di 1 uti on medi urn (SDM) pro .... ’e1j to be a most re 1 i attl e rnedt urn Wl1i eh qive a hi qh f ert i1 itW rates. .onsnOn Hie other 1-, a nd , Ijiff erent pr-otect i ’.le sunstencesqlqcerol, etnulene qlucol and t••1ethanol) V’lere useo atIjiff erent concentrati on s !det Di methul sul phox: Ije (Dt•••1S0) at 10%concentrati on wi th equi 1 i brat ion ti me 1 ess then one mi nute •,,•vasU-I e m 0 s t e ff e c t i ve sub s tan c e. T 11 e Ij i 1 ut e d se m en (1: 3) a s either DROPpeo on cerbone dioxtde ice (-79’:OC) in dn t erent pellet sizes (trom 0.05 - 0.5 m1)and left until frozen and stored in1 i quilj m troqen (-196c,C) or asp: relj in straws end rrozenirnrne1jiat1!d to -1 (JO’:-C in embruo freezer. then stewed in the 1 i qui Ij ni troqen.