Buffaloes are considered in the rural regions as a main source for both meat and milk production. Most mycoplasmas are facultative pathogens giving a disease condition only when their hosts succumbed to stress factors or when their body resistance is impaThe present investigation was carried out on 532 samples randomly collected from both apparently healthy and dise-determination of oalcium, inorganic phosphorus and copperas well as ca/p ratio.1. In buffaloes affected with anestrum: There werean elevation of serum calcium as well as a highly