Immunological studies of Newcastle disease virus in fowls

(1) This investigation to throw light on the role played- .by cell mediated immunity in the proteotion of chioken vaccinated with NDV-tissue culture adapted vaccdrie (1TJ) iV-TCAV) estimated by lymphoc Tte transforI:1o.tion and delayed hypersentitivity testsb in corrolationn with the development of humoral antibodies.- ,”2: The mitogenic dose response curve of both PHA and NDVwere studiedo The .highest :mitogenic ratio in normal :chicken lymphocytes to FrIA was reached 934 at_ dilut.ion of 1:50 and oell concentration of O.5:X.106 lymphosytes/ ml in vitro wholeb blood, culture,’ while in vitro spleen lymphocyte culture was 2133IThe MDV mitogenic to normal chicken lymphoytes in vitro whole blood culture after 120 hours incubatieon was 192 at dilution l:100,’ while in vitro spleen ,v lymphocyte culture reeched 52 at dilution 1:1000.(.;) The mitogenic ratio of vaccino.ted chicken lymphocytes to NDV after 5 days postvaccinntion in vitro whole b1ood culture ranged from 15-16 at dilution 1:100 and Lt10 respectively, While in vitro spleen lymphocyte cu-Iture was reaohed 7 at dilution 1:1000.