Studies on sanitary condition in places newcastle vaccine preparation

Investigations were carried out’ to reveal at what stepduring preparation of Newcastle vaccine the bacteric lcsical contamination may occur.This work proved that the contamination of the discarded harvest-amino-allantoic fluid taken frominfected eggs after incubation) during its preparation was due toseveral rao tor-e (I This was done by comparing the isolatesfrom all possible sources that may introduce contaminantsto the he.rvest , with similar strains isolated, from the discarded harvest1) The isolated organisms in experiIr,lent 1 from egg shellwere Anthracoids, (B. cereus 11.4%, B. coagulanse 8.6%, B.subtilis 6.8%, and B. pumils 4.6%) and Micrococci, Staph.epidermidis 11.2%, Micrococcus roseus 4.2% and Micro. lutus3.2% of samples respectively, excit as saprophytes in nature,2) We can classify the isolated organisms from egg in-terior into:- Salmonellae and S. pullorum which were present in 0.4%respectively,0.4% of examined samples may reachinterior of the egg via infected hens.