This work was done to study the various bacterial species causing intestinal disturbances in infants inSharkia governorate with further assessment and iden-tification of the causative organisms :1- The study included 150 diarrhoeal cases, their ages varied between two months up to five years.2- Rectal swabs of stools werecaLly and then cui tured onmedia. examined microscopi-simple and selective3- Pathogenic organisms were isolated from 78 cases constituting (52%) total isolation rate.Escherichia coli was isolated from 30cases (38.46%) salmonella was isolated from 17 cases (21.79%)shigella from 8 cases (10.25%), Staphylococcus aureusfrom 7 cases (8.97,,/0), proteus from 7 cases (8.57%) and2 for eai.r,;.:, OI6 ormes, 4. % for e.•;rgercj slenghtorcd C aIna.s and ‘428 % for epern onta]y injectc roes0 Positive samp?.os i ti :r.sole ad aLdneys vrere I In calvcsin corz oy e,ahtered anirral.s and 5426 in e::rerner.taJy injo .;rI buffaloes0The ro”ocntae o ecs.,t:ve results of rascDos at :11 2O were of cares, 3 of o:creenoy