Biochemical effect of nicotinic acid on thiamine metabolism in the ruminal contents of the camel

the present work was devoted to study the in v:J..tm effect of d.;1.tfere:o.t amo\.1t1.ts Qf protein free substrate as well as the dfect of :o.i.cotinic acid on t.b.iamine uetsynthesis in rumino.l fluJ.d of Egyptian camel.A total of 150 ruminaJ. fluid samples were co.Ll.cc t eddurinG dry season from. slu~htered camels at Cairo aba.ttoir.W.l. oamels used were matuxe and their age lied betwe6-12 yea:r:s old. Screened rumen content were incubated at39°C and pH 6~G under CO2 Bas for 100 minutes with additionof different amounts of substrate (protein free diet) cont nin8 urea as the only source of dietry ni troc,en.~other series of exp~rjments concerning the eff ect ofdifferent nitocinic acid concentrations on the thialline netsynthesis wa.s applied.Samples were taken from th.e incubating rumina.l fluidlit 0, 30, 60, 120 and 100 minutes to dete:nn.ine the thiomint.cont€Ut, protein-nitroGen and tho volatile futty ucius.The rum.i.uo.l fluid o! camals ’were incubat~ for 60minutes vd-thout ooy treutment, and also with diii erent