Studies on the effect of different Adjuvants on the efficiency of FMD vaccine in farm animals

Different bat ches of lllY.mV vvere prepared on monolayer cult-urea of BHK-21 clone-I) cells, treQted vrith cbloroform and concentrated by PEG (GoOO). The pOeled concentrated FMDVwas assayed for virus infectivity, antigenicity and 140 s.virus particle s • The prepared concentrated Ii’LmlY was imctivated by AEI and proved to have :no viable }I’Ii1DV by innocuity testing itssafety on BRK monolayer cell culilure . Constnnt 8lilOunts of concentrated and inactivated F1IDV wereformulated as vs.ccines using aluminium hydroxide gel/saponin(conventional) ,FreundtS’ incomplete (oil-emulsion) and DEAlS-de.:tru.n ae adj uvants •The formulated vacc i nos proved to be sterile and free from any pathogenic and non~.pathogenic microbial agents •?he ili1111unogenic power of the three batches of FlvID vacc LnasI’ere tested in guinea pigs USilJg the eo bay~ index (Cl) and!Uinea pig protective dose fifty (GP.PDSO). The three batches~d cr in excess or equo.l to 2 10g-IO; ’while the 5.ml •. ~ttle dose of oil-emulsion, conventional o.nd DEAE-dextranijuvanted FMD vacc Lnas had values of 500, 166.7 and 31.25