Studying the passive haemagglutinating antibodies and comparing it with the fluorescent antibody technique in diagnosis of African horse sickness virus disease

In this study the resUlts revealed the followingpOints : The microplate technique of the complement fixationtest by using the partially purified soluble antigenof AHS virus with the collected sera sample ofdifferent animal species Other than equines revealedthe presence of AHS antibody in the sera of suchanimal species. The percentage of the positive serawith the microplate technique of the complementfixation test was 20 in case of donkey sera, 11.8%in case of sheep sera, 10.3~ with regards to goatsera, 6.9~ with regards to buffaloes sera, 4.5with regard to dog sera and 0.93 in case of camelsera. The microslide technique of the agar gel diffusiontest by using the partially purified soluble an~igenof AHS virus showed the presenee of AHS antibody in the tested sera of the different animal species.The percentage of the positive sera were 15.210 in case of goat sera, 15% with regards to cattle sera~11.25% with regards to buffaloes, 9.8~ in case of dog sera and in case of sheep sera. Also it wasobserved from the data that a considerable role wasplayed by animals rqvher tan pquine