Biochemica evaluation of buffalo”s milk enzymatic activity in normal & mastitic animals

r:iastitis has important significance in the economy of milk production, it is the most serious disease problemconfronting the dairy farmer. Several methods for diagnos-ing mastitis have been reported. Bacteriological methods is expensiYe and time consunfng , hence ’the .need ’for simple,sensitive and reliable method sufficient to be applied on alarge scale for herd testing Ls required. Quarter milk samples wer-e collected from 140 lactatingbuffaloes (Bos buba11s) and 62 local breed cows at Edfina veterinary clinic and the clinic of the Faculty of Edfinavet. Med. Alex. Uni versi t y , Bacteriological examination ofsamples collected from apparently normal animals that 18% of buffaloes and 50% of cows wer-e infectedrrith specific microorganisms of mastitis. The most predomin-ant organisms isolated trom buffalo’s and cow” s milk in cases of~stitis (subclinical and clinical cases) were Str. agal-aetiae and Sta ph. Cl ureus •Y.1lk samples collected from buffaloes and cows wer-e ana Ly s edfor determination of:(1) Glutamic oxalacetic transaminase (GOT) (2) Gluta.mic pyruvic transaminase (GPT)13) Alkaline phosphatase (ALP)