Biochemical Studies On Mammalian Bemoglobin With Aspecial Reference To Bat (Rousetius Aegyptiacus) & Reindeer (Rangifer Tarandus Tarandus)

The present work was planned to study the oxygen binding properties of mammalian hemoglobins with a special reference to Bat and Reindeer hemoglobin. The Bat (Rousettus aegyptiacus) had been breed in Cairo,Egypt, the blood samples were collected in isotonic NaCl solution containing 2 mM EDTA. After that the hemoglobin areprepared. Stripped hemoglobin was obtained by passing the haemolysate over a mixed-bed ion exchange column (Dowex Ag The reindeer (rangifer tarandus tarandus) had been breed inBlood samples were collected in isotonic NaCl solution containing 2 mM EDTA, after that hemoglobin areprepared and stripped as in case of bat. Small amount of bloodremain without hemolysis to see the Bohr effect of red blood cells. The hemoglobin from Bat and Reindeer were subject to investigate this parameters: